Testosterone,Free/Total, Serum


To detect an abnormal testosterone level in males and females: In males, to help diagnose the cause of symptoms, such as erectile dysfunction or the inability of your partner to get pregnant (infertility). In females, to help diagnose the cause of masculine physical features (virilization), excess body hair (hirsutism), infertility, or polycystic ovary syndrome (PCOS). In children, to help determine the cause of delayed or early (precocious) puberty, or the cause of genitals that are not clearly male or female (ambiguous genitalia) To monitor testosterone levels in transgender individuals who are undergoing hormone therapy


More about Testosterone free / Total, Serum

The Testosterone blood test measures the amount of testosterone in the blood.

Other names: 
Testosterone test, Testosterone Total

Testosterone is the most important sex hormone in the men. Sex hormones is also referred to as Androgens. It is present in both males and females but is present in higher concentrations in males. In men, it is produced in the testicles and also by the adrenal glands. In females, testosterone is produced by the adrenal glands and the ovaries and most of the testosterone is converted to Estradiol.

Twelve hours before this blood test, do not take multi vitamins or dietary supplement containing biotin or vitamin B7 that are commonly found in hair, skin and nail supplement. If on any medication or treatment please indicate and provide clinical history, if any.

Why Testosterone test?

Since testosterone is the most important sex hormone in males, it is an important test while evaluating infertility in men. The testosterone test is used in males to diagnose erectile dysfunction and infertility. Testosterone is ordered in women when conditions like PCOS is suspected.

The test is also used for individuals who plan to undergo hormone replacement therapy.

Who should get tested for Testosterone?
  • Testosterone test is used to evaluate delayed or early puberty in boys
  • To evaluate decreased sex drive and erectile dysfunction
  • Conditions that lead to hirsutism in women. Hirsutism refers to excess facial and body hair on face and chest. Hirsutism is most commonly observed in women with PCOS
  • To evaluate Ambiguous genitalia in infants
  • Test is also used in the diagnosis of pituitary disorders


Reference values for Testosterone


Guideline for total testosterone biological reference rage based on Tanner stage:

Male Tanner stage Biological reference range (ng/dL)
Tanner stage I (Age 9) < 20.0
Tanner stage II (Age 11) < 7.0 – 149.0
Tanner stage III (Age 13) 7.0 – 762.0
Tanner stage IV (Age 14) 164.0 – 854.0
Tanner stage V (After age 15) 194.0 – 783.0


Female Tanner stage Biological reference range (ng/dL)
Tanner stage I (Age 8) < 17.0
Tanner stage II (Age 9 – 11) < 7.0 – 39.0
Tanner stage III (Age 12) 10.0 – 60.0
Tanner stage IV (Age 13) 8.0 – 63.0
Tanner stage V (After age 15) 10.0 – 60.0



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